Genetic Characterization and Population Structure of Pea (Pisum sativum L.) by Molecular Markers against Rust (Uromyces viciae-fabae) in Newly Developed Genotypes

The understanding of the genetic diversity germplasm any crop is necessary for improvement. Pea (Pisum sativum L.) a very important legume that provides protein and several essential vitamins, carbohydrates, minerals. population structure pea consisted 115 entries Australian accessions 4 Indian varieties used as checks with varying responses severities rust, which were analysed using 31 polymorphic SSR (Simple Sequence Repeats) markers. combination markers revealed 78 alleles present at 32 loci. It was also observed each marker had three an average PIC (Polymorphic Information Content) value 0.272. analysis showed differentiation entries. model-based grouped into sub-populations SP1, SP2, SP3 having 37, 35, entries, respectively 15 admixtures. AMOVA (Analysis Molecular Variance) disclosed there 56% variation among individuals 20% within population. A mean fixation index (Fst) 0.240 exhibited relatively significant in This study basic information to select parental lines developing rust resistant meet ultimate goal sustainable agriculture.